... and denatures other proteins through dehydration. In contrast, the time for each dehydrating step should be sufficiently long to ensure a gradual substitution of water with solvent. PREPARATION OF BIOLOGICAL SPECIMENS . Key words: Tissue,processing,staining,fixing,dehydration,clearing. Dehydration is usually carried out using alcohols (such as ethanol) but these can dissolve certain cellular components such as lipids. A procedure which needs to take place between tissue fixation and the embedding/sectioning of paraffin blocks is tissue processing. I. Paraffin Processing of Tissue. The removal of water is dehydration. Last update on October 3, 2016 under Sample Preparation; Fixation of Tissues. Processing Equipment and Factors Affecting Tissue Processing; Dehydration and paraffin embedding of tissues for histology WOLF D. KUHLMANN, M.D. BOT/MICRO/ZOO 5364 . Tissue processing Tissue and organ preservation (perfusion fixation), tissue processing for microscopy (dehydration, embedding, cutting, mounting, coverslipping) Oral Rehydration Therapy (ORT) a ... Why is Rehydration so Important and How it Works to Save ... individual and the degree of dehydration. Fixation, Rinsing, Dehydration and Embedding steps of Tissue Processing for TEM are discussed along with standard Available Topics ... to help distinguish the components of the tissue. TESTING FOR DECALCIFICATION. FIXATION . DEHYDRATION The first stage in tissue processing is dehydration (the removal of water). Your body depends on water to survive. The aim of Tissue Processing is to remove water from tissues and replace with a medium that solidifies to allow thin sections to be cut. Paraffin Processing of Tissue. Share. Some people are at higher risk of dehydration The duration of the dehydration procedure should be kept to a minimum in order to reduce these effects. The main steps in this process are dehydration andclearing. What Is Histology. The page below is a sample from the LabCE course Tissue Processing in Histology. Microscopic Quality Control of Hematoxylin and Eosin Know your Histology Breast ... Fixation and Tissue Processing Fixation and Tissue Processing Introduction Aim of tissue processing is to remove water from the tissue and replace it with the medium that solidifies to allow thin sections to be cut. Last update on October 3, 2016 under Sample Preparation; Fixation of Tissues. If the tissues are to be embedded in paraffin or plastic, all traces of water must be removed: water and paraffin are immiscible. Tissue processing is concerned with the diffusion of various substances into and out of porous tissues. degree of tissue shrinkage and extraction of other tissue components (differ ential extraction). The dehydration process is accomplished by passing the tissue through a series of increasing alcohol concentrations. Hydration: Why Its So Important. The main steps in this process are dehydration andclearing. Biological tissues must be supported in a Remove 5 ml of used decalcification fluid from the tissue processing vessel; Add ammonium hydroxide dropwise In tissues, water is present in both free and bound forms and needs to be removed before processing can continue.